The ability to easily and accurately edit DNA and nucleotide sequences is a long-time dream of researchers. The dream of the birth and maturity of the CRISPR/Cas9 system has gradually become a reality. The CRISPR/Cas9 system, as the third generation of gene editing technology, is essentially a defense system against bacterial foreign DNA such as viruses. The system works by clustering, regularly spaced short palindromic repeats of crRNA ( CRISPR-derived RNA) that are coupled to tracrRNA (trans-activating RNA) by base pairing to form a tracrRNA/crRNA complex that directs the complex. The nuclease Cas9 protein cleaves double-stranded DNA at a sequence target site that is paired with the crRNA. By artificially designing these two RNAs, it is possible to engineer a sgRNA (short guide RNA), which is sufficient to guide the Cas9 to cut-point DNA. Studies have confirmed that Cas9's genome editing ability is achieved by a short DNA sequence called "PAM" (protospacer adjacent motif). This study reveals two major functions of PAM, explaining why it is so critical for Cas9 to target and cleave DNA sequences that match the targeting RNA. PAM is present near the target site of the foreign DNA, and these target sites in the host genome lack PAM, enabling Cas9 to accurately distinguish between non-self DNA that must be degraded and nearly identical self DNA. In addition, the presence of PAM is also a necessary condition for the activation of the Cas9 enzyme. As a professional solution provider for epigenetics, Amy Jie recommends the Epigentek brand CRISPR/Cas9 Polyclonal Antibody, which is currently the only CRISPR/Cas9 antibody in the world, using a partial polypeptide fragment of the Cas9 protein as an immunogen. The rabbit-derived polyclonal antibody, which has been further optimized for preparation, can be used for multi-antigen recognition in experiments such as IP and ChIP, and can be used for numerous studies in gene editing and the like. The CRISPR/Cas9 polyclonal antibody reacts with human, mouse, rat, rabbit, and goat species and has a broad spectrum of species versatility. In terms of its application, the antibody is powerful and proven to be used in a variety of experimental applications including ChIP, IP, ELISA, WB and IF. It is your perfect companion for research and application of CRISPR/Cas9! As a new research and development area of ​​genes, Wuhan Aimeijie also provides you with a full range of genetic manipulation related products including CRISPR/Cas9 system. Epigentek Group is the world's leading developer and supplier of innovative technology and epigenetic research products. The company has developed a comprehensive product portfolio of more than 400 patented products to provide comprehensive system solutions for epigenetic research and new drug development. As the general agent of Epigentek Group's China region, Amy Jie introduced it to China as its regional agent in China and will provide customers with complete epigenetic solutions, including analytical kits for antibodies, antibodies and related Products such as inhibitors/antagonists. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions. Detailed address http:// Zhoushan City Shuangying Aquatic Products Co., Ltd.  , https://www.shuangying-aquatic.com
By mutating the Cas9 sequence and merging with different functional domain proteins, different targeted gene manipulations such as DNA cleavage, transcriptional repression, regulatory factor localization, and fluorescent tag localization are achieved. The CRISPR/Cas9 system also has the following advantages: First, the CRISPR/Cas9 system is available in more locations. In theory, every 8 bases in the genome can find a position that can be edited with CRISPR/Cas9. It can be said that this technology can operate on any gene. Secondly, the CRISPR/Cas9 system is more scalable. For example, the Cas9 protein can be modified so that it does not cut the DNA double strand, but only the single strand is cut, which can greatly reduce the difference caused by cutting the double strand. The risk of chromosomal variation caused by the end of the source. Third, and more importantly, the use of the CRISPR/Cas9 system is extremely convenient and can be done in a few simple steps, and almost any laboratory can work without the assistance of commercial companies like ZFN and TALEN. carry out. Due to its many outstanding features and wide application prospects, CRISPR/Cas9 has become a hot research and application field. 
