Genmaicha ("Brown rice tea"), is a Japanese brown rice green tea consisting of green tea mixed with roasted popped brown rice. It is sometimes referred to colloquially as "popcorn tea" because a few grains of the rice pop during the roasting process and resemble popcorn, or as "people's tea", as the rice served as a filler and reduced the price of the tea, making it more available for poorer Japanese. Today all segments of society drink genmaicha.It was also used by people fasting for religious purposes or who found themselves to be between meals for long periods of time.The sugar and starch from the rice cause the tea to have a warm, full, nutty flavor. It is considered easy to drink and to make the stomach feel better.Tea steeped from genmaicha has a light yellow hue. Its flavor is mild and combines the fresh grassy flavor of green tea with the aroma of the roasted rice. Although this tea is based on green tea, the recommended way to brew this tea is different: the water should be at about 80–85 °C (176–185 °F), and a brewing time of 3–5 minutes is recommended, depending on desired strength.
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The above figure shows that when the blood cell count plate is at high cell density, the counting result deviates from the actual cell density, while the image-based cell counter has a large deviation at multiple concentrations. This indicates that the Coulter counter-based cell counters (Coulter counter and Scepter cytometer) are superior in counting accuracy to the other two cytometers. (sample is common COS7 cells)
[reproducibility comparison]
As can be seen from the above figure, the repeatability of the cellulometer based on the Coulter principle is significantly better than that of the blood cell counting plate and the image-based cell counter. (samples are 19 cell lines)
[Shortcut]
As can be seen from the above figure, the counting time of the traditional blood cell counting board is much longer than the other two counting. The Coulter principle counting method is more stable than the image-based cell counting method, and the counting time is about half of the latter. (samples are common SF9, MCF7, HEK293 cells)
[Results]
In summary, the cell counter based on the Coulter principle leads the image-based cell counter and the hemocytometer in terms of counting accuracy, reproducibility of results, and counting speed.
Common cell counter performance competition
Since traditional blood cell counting plates have been unable to meet the needs of high-speed cell research, there are more and more devices for automatic cell counting on the market. Commonly divided into two categories: Automated vision-based counters and cell counters based on Coulter's electrical impedance principle. The main difference between the two is that the former scans the image in the field of view of the instrument, and relies on the set upper and lower limit cell size for image recognition, while the latter calculates the number of cells according to the potential change caused by the cell through the small hole (for the Coulter principle, see Here), the two principles are different, so the results obtained are also very different. The following is a comparison of the results of counting, repeatability and quickness of various cell counters. It is recommended that readers choose the cell counter that is suitable for your use according to your own needs.
[ Accuracy comparison] Figure 1. Comparison of count results and actual values ​​of various cell counters at different cell concentrations
Image-based cell counter stands for: Invitrogen Countess, Bio-rad TC-10
Cell counting instrument based on Coulter principle representative products: Merck Millipore Scepter, Countler Z2