Photogenetics "reconstructs" cardiomyocytes

:2010-11-12


The researchers found that light stimulates genetically engineered heart cells, which also allow the heart to be stimulated in the same way, which will help with normal heart function research and may open a new door: in rodents Light is used as a pacemaker in animal research. The new results were published in the journal Nature-Methodology, which was published online a few days ago.
Optogenetics is an established method that controls cell behavior by light by means of genetically encoded light-sensitive proteins from micro-tissues. The blue light pulse can open the positive ion light sensitive channel ChR2, which can also stimulate the transmission of electronic signals when the channel is expressed on the surface of the stimulated cells. Neurons that overexpress ChR2 are a common tool in neurobiological research because they can be selectively activated. Philipp Sasse and colleagues extended the use of ChR2 to other stimulated cells in the body, such as heart cells.
They expressed ChR2 in mouse cardiomyocytes and stimulated the growth of these cells with light in test disks and transgenic mice. Light pulses promote the localization and duration of current in cardiac cells.

Source: Science Times

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