Many mothers have insufficient breast milk after giving birth, making milk powder a must, but some infants and young children are easy to spit milk, indigestion, and diarrhea. The important reason is that infants have low enzyme ability to synthesize and decompose food. Nanjing University of Technology, Professor Ying Hanjie presided over the "Key Technology Breakthrough of Yeast Nucleic Acid Manufacturing and High-end Application in the Industry", which fundamentally solved this problem. Recently, the project won the second prize of the National Technology Invention Award. It is understood that the production methods of nucleotides include chemical synthesis and RNA enzymatic hydrolysis. Many Western developed countries have clearly defined that the added nucleotides in infant foods must be derived from yeast RNA hydrolysates. However, this method has three difficulties: low enzymatic yield, lack of efficient separation process, and many raw material RNA impurities. After 12 years of research, Professor Ying Hanjie has made three breakthroughs in the core key technologies: First, invented the high-yield nuclease P1 strain and pioneered a new technology of immobilized continuous enzyme production with self-added value and self-repair function. The problem of low nuclease P1 activity and many hybrid enzymes was solved. Secondly, a new separation medium was designed. For the first time, high-yield and high-purity stable separation of 4 nucleotides was achieved by primary resin separation. Invented the new composite crystallization system and equipment for reaction-crystallization and alcohol precipitation and salting out, which made the product quality improved significantly and the process flow was greatly shortened. Thirdly, the high-nuclear yeast breeding technology based on the combination of cell fusion and metabolic evolution was invented. The high-efficiency continuous culture technology solves the problem of low RNA content in yeast, and pioneers a new combination of intracellular nucleic acid directed extraction, which solves the problem of low RNA purity. The new technology in the project has been certified by domestic and foreign authorities such as China, the United States, and Switzerland. The domestic market share is about 70%. At the same time, the products have entered internationally renowned companies such as Europe and the United States, and the world's four tanks of high-end milk powder with added nucleotides. One can use this product, breaking the monopoly of domestic high-end infant milk powder market by foreign brands, and enhancing the international image of China's food additive industry.