Hepatology article: reveals that small RNAs in the host are involved in the regulation of hepatitis B virus expression

Hepatology article: reveals that small RNAs in the host are involved in the regulation of hepatitis B virus expression

Hepatitis B virus (HBV) infection can cause acute and chronic inflammation of the liver. Acute infection is generally characterized by self-limiting hepatitis. In addition to fulminant hepatitis, it is less harmful to the human body; while chronic infection is closely related to the occurrence of cirrhosis and hepatocellular carcinoma (HCC). There are about 350 million HBV carriers in the world, and the proportion of liver cancer that is eventually developed is as high as 25%. More than 1 million patients die of HBV-related liver cancer each year. China is a country with a high prevalence of HBV. HBV infection is an important infectious disease that seriously endangers the health and quality of life of the people.

MicroRNA is a kind of small non-coding RNA, which can regulate gene expression by degrading target mRNA and inhibiting translation of target mRNA. It plays an important role in the growth and development of animals and plants, the occurrence and development of tumors, and the development of infectious diseases. Recent studies have found that microRNAs are involved in the replication of HCV, HIV, Epstein-Barr virus, Plum pox virus, CMV virus, Kaposi''s sarcoma-associated virus and other viruses in host cells, regulating host cell-virus interactions. It affects the occurrence, development and outcome of the disease and may become a new target for antiviral therapy. Prof. Cheng Jing's research group and researcher Guo Yong, together with Professor Wei Lai of Peking University People's Hospital and Professor Huang Ailong of Chongqing Medical University, used the biochip platform to combine functional studies to find that miR-372/373 small RNA can regulate host cells. Transcription factors promote HBV expression. This study provides a new direction for the study of HBV expression regulation by correlating microRNAs and transcription factors, which are very important regulators in cells, and has important theoretical significance.

The above research of Boao BioCrypt® Mammalian miRNA chip service and Real time RT-PCR service was completed at Boao Biosystems Co., Ltd.

Original summary:

MicroRNAs-372/373 promote the expression of hepatitis B virus through the targeting of nuclear factor I/B

MicroRNAs (miRNAs) play important roles in the posttranscriptional regulation of gene expression. Recent evidence has indicated the pathological relevance of miRNA dysregulation in hepatitis virus infection; however, the roles of microRNAs in the regulation of hepatitis B virus (HBV) expression are still largely Unknown study We identified that miR-373 was up-regulated in HBV-infected liver tissues and that the members of the miRs-371-372-373 (miRs-371-3) gene cluster were also significant co-up- Regulated in HBV-producing HepG2.2.15 cells. A positive in vivo association was identified between hepatic HBV DNA levels and the copy number variation of the miRs-371-3 gene cluster. The enhanced expression of miRs-372/373 stimulated the production of HBV proteins and HBV core-associated DNA in HepG2 cells transfected with 1.33HBV. Further, nuclear factor I/B (NFIB) was identified to be a direct functional target of miRs-372/373 by in silico algorithms and this was subsequently conf Immed by western blotting and luciferase reporter assays. Knockdown of NFIB by small interfering RNA (siRNA) promoted HBV expression, isolated rescue of NFIB attenuated the stimulation in the 1.3xHBV-transfected HepG2 cells. Conclusion: Our study revealed that miRNA (miRs-372 /373) can promote HBV expression through a pathway involving the transcription factor (NFIB). This novel model provides new insights into the molecular basis in HBV and host interaction.

Original source: http://onlinelibrary.wiley.com/doi/10.1002/hep.24441/abstract;jsessionid=6B0DBCF4745EF5CB4D33C995287182BA.d01t04

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