Critical Care,Intensive Care,Critical Care Nursing,Critical Care Medicine Zhejiang Hisern Medical Technology Co., Ltd , https://www.hisernmedical.com
Preparation: Just start the measurement by turning on the power switch on the back of the instrument before starting the measurement, without preheating.
1. Determination of nucleic acid concentration (including dsDNA, ssDNA, RNA, Oligo)
1. For example, if the sample to be determined is dsDNA (eg: PCR product), press the key "7 / dsDNA"
(If the sample to be tested is ssDNA, eg: the first strand cDNA product synthesized by reverse transcription, press the key "8 / ssDNA" accordingly;
If the sample to be tested is RNA, press the key "9 / RNA";
If the sample to be tested is Oligo (oligonucleotide), eg: PCR primer, press the button "6 / Oligo". )
2. Place a blank control into the sample well. Note: The blank control is a blank, not all cases are water. (For example, if the DNA product is dissolved in Tris solution, Tris solution should be used as a blank control.)
3. Press the "Blank" button;
4. The instrument records a blank control and sets it to 0.000A;
5. Place the first sample into the sample well;
6. Press "Sample";
7. The instrument displays the absorbance and concentration values ​​of the first sample, as well as other relevant reference ratios;
8. Insert the second sample directly;
9. Press "Sample";
10. The instrument displays the absorbance and concentration values ​​of the second sample, as well as other relevant reference ratios;
11. The measured values ​​of each sample are automatically stored in the machine. The method of viewing the results is as follows:
(1) Press the key "./ Function"
(2) Select “DISPLAY-RESULT†and press Enter to view the measured value record for each sample (this unit can store the measured value of 100 samples).
Set the dilution of the sample (1) Press the button "Dilution";
(2) Enter the sample volume and diluent volume and press Enter to confirm. 
Second, the direct determination of protein concentration (measured at 280nm)
1. Press the key "4 / Protein";
2. Place a blank control into the sample well;
3. Press the "Blank" button;
4. The instrument records a blank control and sets it to 0.000A;
5. Place the first sample into the sample well;
6. Press "Sample";
7. The instrument displays the absorbance and concentration values ​​of the first sample, as well as other relevant reference ratios;
8. Determined in sequence, the measured value of each sample will be automatically stored in the machine, and the measurement results can be viewed.
3. Indirect determination of protein concentration chromogenic method (Bradford, Lowry, BCA)
1. Press the key "1 / Bradbord" or "2 / Lowry" or "BCA" to select the protein color reaction method;
Note: The Bradford button is pressed twice, each time displaying a different measurement range.
2. Set the standard sample number, number of measurements, and concentration range for the standard curve. Press the "Parameter" button to select and parameterize with the up and down keys.
3. Place a blank control into the sample well;
4. Press the "Blank" button;
5. The instrument records a blank control and sets it to 0.000A;
6. Place the first standard or sample (if you need to use the stored standard curve, you can directly measure the sample) into the sample well;
7. Press "Sample" or "Standard";
8. Determine the standard or sample in turn, and the measured value of each sample will be automatically stored in the machine to view the measurement results.
IV. Determination of OD600 bacterial growth density 1. Press the key "5 / OD 600";
2. Place a blank control into the sample well;
3. Press the "Blank" button;
4. The instrument records a blank control and sets it to 0.000A;
5. Place the first sample into the sample well;
6. Press "Sample";
7. The instrument displays the absorbance and concentration values ​​of the first sample;
8. Determine in sequence, the measured value of each sample will be automatically stored in the machine to view the measurement results.
Five, note:
1 In order to minimize the effect of particles on the test results, the absorbance of the nucleic acid is required to be at least greater than 0.1 A, and the absorbance is preferably between 0.1 and 1.5 A. The interference of the particles in this range is relatively small and the result is stable. The concentration of the sample should not be too low or too high.
2 Mixing should be sufficient, otherwise the absorbance value is too low, and even a negative value occurs;
3 There should be no air bubbles in the mixed liquid, and there is no suspended matter in the blank liquid, otherwise the reading will drift drastically;
4 The blank and the sample must be tested using the same cuvette, otherwise the difference in the measured concentration results is too large;
5 The conversion factor and sample concentration unit selection should be consistent;
6 Cannot use the cuvette with window wear;
7 The volume of the sample must reach the minimum volume required by the cuvette (50 ul);
8 Normally sized samples can be tested with a 10 mm path length. For high concentration samples, simply rotate the cuvette 90° and use a slightly shorter 2 mm path.
critical patient care system
Eppendorf Protein Nucleic Acid Auto Analyzer Operating Instructions and Precautions
Instructions: