Organizational library frozen sample management process recommendation

Organizational library frozen sample management process recommended Shanghai Wangge Zhou Xuexun

one. Material collection and numbering/labeling section In this section, the tissue and blood are taken, and a barcode label is produced to dispense the sample.
1. Drawing list: The surgeon will inform the sample bank the next day's sample on the day before surgery according to the clinical case, and indicate the number and type of blood samples to be taken in the doctor's order. The sample library prepares the sample extraction form in advance according to the sample of the next day, and pre-defines the serial number. The information on the sample extraction form may include, but is not limited to, a subset of the following:
A. Individual serial number
B. Basic information about the individual: such as hospitalization number, pathology number, surgery department, surgeon, patient name, gender, etc.
C. Sample characteristic information: such as organ name, sample type (tumor, adjacent to cancer, normal, lymph node, polyp, cyst wall), sample properties (primary/recurrence/metastasis), etc.
D. Sample collection information: such as sample size, volume, number of tubes, preoperative / postoperative, etc.
E. Diagnostic information: such as clinical diagnosis, pathological diagnosis, degree of differentiation, UICC staging, ICD10/ICDO-3 code, etc.
F. Treatment information: such as radiotherapy, chemotherapy, surgery, etc.
G. Biosafety information: with/without contagious
H. Time information: acquisition time, sample ex vivo time, low temperature time, etc.
I. Staff information: material pickers, recorders, etc.
J. and remarks.
2. Tissue sample: Collect tissue samples from the operating room and fill out the sample list. Part of the column is registered on the organization library cryopreservation management software. If the hospitalization number is entered, the software automatically transfers the individual information (basic information, clinical information, etc.) of the case from the HIS.
If part of the information is input, the system automatically generates the individual number consecutive to the last time, such as selecting the organ name, sample type, sample storage mode, and number of tubes, and the software generates the sample number according to the rule, such as 1012345D19TF1~1012345D19TF4, in the corresponding sample. In the information bar, enter the characteristics of the sample collection information of each tube, click Save to save all the information into the database, and automatically generate sample barcode information on the liquid nitrogen barcode label of the operating room. The ex vivo tissue samples were stored in a liquid-resistant nitrogen storage tube, and after labeling, liquid nitrogen was immersed in 30 minutes. After the sample collection is completed on the same day, the sample is transferred to the laboratory using a small liquid nitrogen transfer tank or ice box.
[The number of tissue samples is named 1012345D19TF1, which represents the paracancerous sample of the D19 organ collected by the 1012345 disease (T
Tumor, P-cancer, N normal, L lymph node, Y polyp, C wall) F-mode (Frozen Tissues/OCT/
The first copy of RNAlater/DNA/Paraffin embedding, etc.). 】

3. Blood samples: Each department sends a blood sample to the sample bank, attaches a blood sample table, fills in the information of the material list, and the number of tubes on the material list is left blank. According to the names of different primary lesion organs, the samples and the materials are classified, and each type is discharged in order.
a. Print label: import the individual information into the organization library frozen storage management software by inputting the hospital number, and then input the serial number and the organ name on the material list into the software, and input the preset number of tubes corresponding to different components, and automatically generate according to the rules. Sample numbers, such as 1012345D19BP1 to 1012345D19BP5, automatically generate a -80 degree label on a laboratory bar code printer.
b. Attach the label to the EP tube and arrange it on the centrifuge tube holder in sequence.
c. Centrifuge the blood sample for centrifugation, fill each batch with a predetermined number of tubes, store it in a pre-arranged centrifuge tube, and check the number when depositing.
[The above method is a procedure for labeling and then dividing blood. It is also possible to consider first labeling and then labeling. The process is as follows: centrifuge the blood sample for centrifugation, store the different components in the EP tube as needed, and sort by sample type. The individual pipe number on the material list is manually written on the EP pipe cover, and the exact number of pipes of different component samples is registered on the material list. After completion, according to the information on the material list, the relevant information is input through the software, and the barcode label with resistance of -80 degrees is automatically generated according to the rules. The entire batch of centrifuge tubes are labeled in order and then stored at -80 degrees for storage. 】
[The blood sample number is named 1012345D19BP1, which represents the P plasma of the B blood sample of D19 organ cancer collected by the 1012345 disease (Plasma/ Serum / White Blood Cells/ Erythrocyte/Thrombolytic / DNA/ RNA/whole Blood/etc.) One. 】
Note: When storing cell suspensions, secretions, excretions and other types of samples, the numbering rules refer to Schedule 2.

two. Sample storage section In this section, the barcode storage tube is stored in the barcode storage box according to the pre-assigned position information. Simultaneous storage on the software is achieved by scanning the bar code of the cryotube.
4. Sort the sample in the -80 degree horizontal refrigerator, store the barcode storage tube in the barcode storage box according to different organs, and check the barcode between the frozen storage tube and the frozen storage box. The positional relationship enables the synchronization of information on the software. When the frozen storage box is stored in the corresponding position of the -80 degree horizontal refrigerator, the scanning of the barcode of the frozen storage box is also realized by software on the synchronous storage.
It is recommended to store the samples according to the name of the original organ. Each frozen box contains the same type of sample of the same organ. Different types of samples are stored in different colors of the frozen box (such as serum, plasma, white blood cells, Frozen tissue, RNALATER, cellomics).
For easier use and to reduce the number of freeze-thaw cycles during use, it is recommended that different copies of the same type of sample from the same organ be stored in different freeze-storage boxes.
It is recommended to pre-plan the storage location of the frozen storage box. Before the frozen storage box is placed in the horizontal finishing refrigerator, the label has been pre-applied, including the organ name, sample type and position number. After the frozen storage box is full, a label can be attached to indicate the serial number of the internal cryotube.
5. After the frozen storage box of any disease in the horizontal finishing refrigerator is full, transfer it to the pre-planned vertical-80 degree ultra-low temperature refrigerator for long-term storage (if different shelves are planned for different organs) When the frozen storage box is transferred, the positional movement of the synchronization on the software is realized by scanning the barcode of the frozen storage box.

three. Information association
6. Import the individual information of the sample:
The individual information of the sample can be directly imported through the HIS association in the link of the second and third points above. If the import of the HIS interface is not possible, you can import it by EXCEL format periodically.
The information corresponding to the individual source of the sample (basic information, medical history, course of disease, diagnostic information, pathological information, test information, treatment information, electronic case information, etc.) is obtained from the hospital information system (HIS) by the hospital number or pathology number. The xls" (EXCEL file format) is exported, and then the relevant information is imported into the cryostat management software, and the number on the cryotube is associated with the information of the sample individual source by the hospital number.
7. Enter the post management information for the sample into the system. The relevant information of the sample, such as treatment results, family data, epidemiological data, follow-up data, etc., can be entered on the cryopreservation management software by direct registration or interface import.
8. Matching of tissue/blood samples: Set the relevant columns in the cryopreservation management system, such as the visit card number, hospital number or ID number, etc. If a sample has both blood and tissue, the system automatically matches the sample and establishes information. Associate and prompt to match the number of samples.

four. Outbound management and daily operations
9. Several details in the use and management of the sample
1) Through the frozen management software, query, statistics, analysis and inbound and outbound management of various predetermined conditions.
2) When there are more vacancies in the sample box in the refrigerator, the storage interface of the corresponding frozen storage box is displayed in different colors on the main interface of the computer, and the corresponding frozen storage box can be managed in parallel, and the empty position can be sorted for new ones. Samples are deposited.
3) Fast information processing through barcode scanning when out of the warehouse.
4) Set account level permissions, set the importance permission level for the sample, and set the permission level for some information fields of the sample. Permissions are tied to the sample importance level in addition to the operational function limits. For example, the on-site material account can only make labels and input basic information; the sample manager can query the sample information and perform the warehousing operation, and can also execute the library; the sample library supervisor account can statistically analyze the sample database information and approve The authorized access account can query the number of existing samples in the library that meet certain basic information, or see the basic information of the sample, but cannot see the privacy field such as name.
10. Sample library identification It is recommended to attach the magnetic frozen shelf simulation logo to the outer door of the refrigerator and fill in the information of the frozen storage box at the corresponding space. For example, the name of the sample in the frozen box, the number of the frozen box, and the start and end of the sample number in the frozen box.
In this way, the basic situation of the internal sample can be seen at a glance before opening the refrigerator door.
Note: [Depending on the final experimental requirements of some samples, it may be necessary to design different storage processes, such as some samples that require protein experiments, directly stored in a liquid nitrogen tank or a -150 degree refrigerator, without going through the finishing of the refrigerator; For example, for samples that require cell and tissue culture, in order to ensure the quality of the sample, it needs to be stored in a -40 degree refrigerator before being transferred to -80 and then stored in a -150 degree ultra-low temperature freezer, or frozen using a program freezer.
Fives. Sample save icon
A. Organization numbering rules and labels

B. Peripheral blood numbering rules and labels

Label type: BRADY THT-163-499-3-IP Rectangular shape is 25.4mm*9.525mm, round diameter 9.525mm
C. Label Description: In the above scheme design, barcode printers and labels use US BRADY products. The label is recommended to use THT-163-499, which can be used for -196 degrees. The rectangular part of the label can print barcodes and numbers, unit logo, and information items of several samples (such as organ name, ICDO-3 number, sample type, collection date, etc.), which can be attached to the side of the cryotube and centrifuge tube. Wall, the dot part of the label can print the serial number, the organ name and the date of collection. It can be attached to the top of the cryotube and the centrifuge tube for easy check. For numbering rules, see Schedule 2 or visit
D. The situation after the bar code label is attached to the cryotube/centrifuge tube is as follows:

E. Label the frozen box and indicate the location number of the frozen box.

F. According to the sample type, carry out the storage box of the frozen storage box.

G. Different parts of the same type of the same organ are stored in different cryoboxes. Different types of the same organ are divided into different color frozen boxes. All the boxes of the same organ are stored in the same frozen shelf, and the horizontal ultra-low temperature in the sample processing room. After finishing in the refrigerator, after a certain box is full, transfer it to the large vertical ultra-low temperature refrigerator in the sample storage room.

H. Vertical ultra-low temperature refrigerators are pre-planned according to different organs.
All the frozen storage boxes placed in the horizontal refrigerator have been pre-labeled, and the label contains the location information of the cryostat that will eventually be stored in the ultra-low temperature freezer.

Annex I: Basic information on the organization of the library cryopreservation container. The common form and storage temperature of tissue storage cryopreservation tubes.
Tissue: cryotube storage (more than internal cryotubes), often stored in -80 degrees / -150 degrees refrigerator or liquid nitrogen, there are also many units to partially infiltrate RNAlater and store at -30 degrees or -80 degrees Conditions, in addition to OCT embedding, wax blocks and other tissue preservation patterns. Try to keep the T/P/N samples in three forms as far as possible.
It is recommended to use a cryotube, a set of TPN is kept in the -150 degree refrigerator, two sets of TPN are kept in the -80 degree refrigerator, and one set of TPN is retained in the RNAlater
Blood: Store in a cryotube or centrifuge tube and store at -80 °C.
The blood should be separated and stored separately in the form of blood components. Plasma, serum, white blood cells, and red blood cells, buffy coat, and lymphocytes are also preserved. It is recommended to store 5 serums in 5 ml centrifuge tubes, 5 plasmas, and 1 to 2 lymphocytes. From the volume of the sample tubes, there is an increasing trend of segmentation. This trend is also to maximize the convenience of sample use. And to improve the quality of the sample (reduced the number of freeze-thaw cycles), currently stored in the 0.5-2ml sample tube.

two. The common form of the cryopreservation box of the tissue library The cryopreservation box is the most basic container for storing the sample tube. Choosing a frozen storage box can bring convenience to the storage sample, and can also reasonably utilize the storage space of the refrigerator, so it is important.
Since the inner space design of the current mainstream ultra-low temperature refrigerator/liquid nitrogen tank is designed based on the 2-inch cryopreservation box, the size of the selected cryostat box should be as standard as possible: 132*132*52.
Under the conditions of this size, there are two types of cryopreservation boxes, one is 9*9 and the other is 10*10. The 9*9 specifications are the most common and are suitable for 1.5-2ml samples of various specifications of various brands. tube. The 10*10 is only suitable for some brands of internal 2ml cryotubes, as well as some special 1.5ml external coils. You need to pay attention to this when selecting a frozen box. 0.5ml centrifuge tube corresponding to the cryostat, in the case of 10*10 size, the length and width dimensions are the same as the standard 2ml cryobox, and the height can be reduced to 2/3 to increase the internal cryostat capacity. From the perspective of frozen storage box materials, there are mainly paper, PP and PC materials on the market. The PC plastic freezer box is compatible with refrigerators and liquid nitrogen tanks. Unfortunately, the cost is too high. The PP plastic freezer box will be brittle under liquid nitrogen conditions. The paper freezer box can be used for liquid nitrogen, but it is applied to liquid nitrogen liquid phase. Need to punch holes beforehand.
The conventional paper cryostat is not waterproof. The patented clamshell cryobox of Shanghai Wange Scientific Equipment Co., Ltd. not only solves the waterproof problem, but also solves the problem of numbering. The clamshell design also solves the problem of difficulty in opening the lid under low temperature conditions. It is currently the best paper frozen box. At present, this type of cryopreservation box is used in large-scale tissue biological sample banks in China.

three. Common forms of frozen storage boxes in the refrigerator
1. Method for placing a frozen storage rack in a vertical ultra-low temperature refrigerator
2. The method of placing the frozen storage box in the vertical freezing shelf:

3. Storage of frozen storage boxes in horizontal low temperature refrigerators

4. Selection of the frozen shelf:
The conventional stainless steel cryopreservation frame is made of SUS-304 or SUS-201 with a thickness of 0.4-1.0mm stainless steel. The type is divided into a drawer type freeze storage frame and a frame type frozen storage frame. Because of the difference in stainless steel materials and processes, the price gap is relatively large.
The Avantech brand CryoMaxTM series of freezers are all available in SUS-304 in three series to cover a wide range of needs.
The IR conventional series of freezers adopt the same mold as the imported freezer. The 0.75mm thick SUS-304 stainless steel is used to punch and form the back plate. It is the best frozen storage rack in China.
The IR-CN series of freezers are made of 0.4mm thick SUS-304 stainless steel and are flanged for the slit. It is an affordable lightweight storage rack that is more resistant to corrosion than the SUS-201 frozen storage racks that are commonly found on the market.
The IR-D series of freezers are drawer type freezers made of a mixture of 0.4mm-1.0mm materials.

four. Ultra-low temperature refrigerator considerations
1. Refrigerator type.
Ultra-low temperature refrigerators are available in vertical and horizontal styles with temperatures of -80 degrees and -150 degrees. At present, the -150 degree refrigerators on the market are horizontal. The advantage of the horizontal refrigerator is that the air-cooling of the door is small, the disadvantage is that the floor space is large, and the vertical refrigerator is just the opposite. Therefore, in the case of large-scale database construction, it is recommended to use a vertical refrigerator for large-scale storage, while a horizontal refrigerator is used for finishing refrigerators that often open and close doors.
2. Area planning.
Due to the construction of the organization library, there will be more and more refrigerators, so when choosing a refrigerator, you need to plan a single sample of the floor space. There are three factors that will be related to this
A. Refrigerator insulation foaming material: The refrigerator with VIP foaming technology is more advantageous for building the warehouse. The technology reduces the outer wall of 140mm thick to 70mm thick, which makes the refrigerator footprint greatly reduced.
B. The larger the refrigerator capacity, the smaller the footprint of the single specimen.
C. The more compact the interior space design, the smaller the footprint of the single specimen.
According to the above three factors, the SFP-branded MDF-U73V is currently the smallest in the world. It can hold 528 standard freezers in less than 0.9 square feet. About 30% smaller than the remaining samples of the same brand of the same type
3. Electricity and cost factors.
Since the 500-litre refrigerator and the 700-litre refrigerator have almost the same power consumption, a large-capacity refrigerator is more cost-effective for the power consumption of a single specimen. In terms of cost, the cost of a single-sample flat-panel device for a large-capacity refrigerator is lower than that for a small-capacity refrigerator, and the power consumption for 10 years is also low.
4. Safety protection device.
The safety protection of the refrigerator involves four contents: 1 is the protection when the power is off 2 is the protection when the power is restored after the power is cut off 3 is the voltage protection during the operation 4 is the use mechanism
1) There are generally two ways to protect against power failure: A. UPS B. LCO2/LN2 Auxiliary Cooling Unit UPS is currently costly and is not used on a large scale. After a certain number of years, there may be a UPS method to charge the UPS at night. During the day, the UPS supplies power to the equipment and uses the difference between the peak and the valley. According to the current UPS and electricity cost, this method is not economical.
The CO2/LN2 auxiliary cooling device, also called the backup refrigeration system (BACKUP SYSTEM), adopts an auxiliary cooling method in which the mechanical temperature sensing device activates the opening of the electromagnetic valve when the temperature rises in the refrigerator, and injects liquid CO2/liquid N2 into the refrigerator. The auxiliary cooling system can be maintained for about 10 hours in case of equipment failure or power failure. The specific length will vary according to the set temperature and cylinder capacity. CO2 auxiliary cooling is suitable for -80 refrigerators. Under normal circumstances, the tank can be maintained at -65. ~-70 degrees or so, and LN2 is suitable for -150 degrees ultra-low temperature refrigerator, which can keep the inside of the box below -130 degrees. The CO2 auxiliary cooling unit uses a liquid CO2 cylinder with a siphon, and the LN2 auxiliary cooling unit uses a self-pressurized liquid nitrogen tank that has a natural loss of LN2.
2) When the power is restored after the power is cut off, because the starting current of the refrigerator compressor is large, when a batch of refrigerators are restarted at the same time, it will cause a secondary impact on the power load, so it is necessary to set different delays for each refrigerator. start up. At present, there are refrigerators on the market that have their own delay setting function.
3) In areas where voltage is unstable, a voltage regulator is required.
4) The use mechanism involves padlocks, electronic code locks, refrigerator temperature monitoring and alarm system, sample room access control system, and sample information security mechanism.
5. Refrigerator recommended common frozen storage box to save sample tube situation comparison table

Recommend the most suitable refrigerator for building a library: Sanyo MDF-U73V
Sanyo MDF-U73V stacking of frozen storage racks (recommended to use the refrigerator, VIP foam and compact design make this model a single sample area the world's smallest):
The first layer is equipped with 6 IR-220s and the second layer is equipped with 6 IR-224s.
The third layer is equipped with 6 IR-220s and the fourth layer is equipped with 6 IR-224s.
Place the frozen storage box in the freezer
IR-220 can hold 20 standard 2 inch freezer boxes
IR-224 can hold 24 standard 2 inch freezer boxes

V. Suggestions for positioning numbering rules for vertical/horizontal refrigerators and liquid nitrogen tanks

The position information of a specimen will be expressed by AA-BB-CC-DD.
Where AA is the refrigerator number
BB is the row and column number of the freezer shelf in the refrigerator
CC is the row and column number of the frozen box in the freezer
DD is the location number in the freeze box
2. The position information of the horizontal refrigerator horizontal refrigerator is also similar to that of the vertical refrigerator. The difference is that the height of the frozen storage rack in the horizontal refrigerator is expressed by the square in the box. Please refer to the following:

3. Liquid nitrogen irrigation

The position information of a specimen will be expressed by AA-BB-CC-DD.
AA is the number of the liquid nitrogen in the main interface of the entire refrigerator / liquid nitrogen irrigation.
BB is the number of the freeze holder. You can select the number of A.....Z or the number of 01...99.
CC is the number of the frozen box in the freezer. The numbering specification is from bottom to top, from 01 to 99, because liquid nitrogen tanks are generally placed from bottom to top.
DD is the number of the specimen in the freezer box. The freezer box is universal and is the same as the freezer box in the refrigerator.

Annex A: Status of domestic and foreign sample banks Domestic biological sample banks. From the perspective of storage containers, the main storage methods currently used are the preservation of ultra-low temperature refrigerators, some of which are preserved by liquid nitrogen tanks, and a small part of the robots are automatically tested. Sample library (-80). In recent years, the international large-scale sample library has been gradually increased by the automatic storage of the robot arm.
The preservation of the -80 degree refrigerator or liquid nitrogen depends mainly on the storage time and the final experimental direction. It is generally believed that -80 degrees corresponds to 5-10 years of storage, and liquid nitrogen conditions correspond to long-term storage. From the research direction of the sample, DNA preservation and RNA storage with RNA protectant are reliable at -80 degrees, and if protein is studied, try to store it with liquid nitrogen. The storage of the robotic arm sample library is currently -80 degrees in the device. Due to the high cost comparison, it is mainly used for high-risk samples and very precious samples.
From the form of the sample tube, the main sample tube is 1.5-2ml cryotubes, and there are also a number of 0.5-1.5ml centrifuge tubes, and a special 0.5-1.0ml cryotube for the robotic arm library. For long-term preservation of samples, cryotubes are more reliable.
There are two types of cryotubes, one is internal rotation and the other is external rotation tube. In the same volume, the outer diameter of the inner coil is smaller than the outer diameter of the outer coil, so the 2ml inner coil can be stored in 10*10 in a standard 2 inch freezer box (132*132*52mm), while 1.8ml external rotation The tube can only be stored in 9*9. The anti-pollution performance of the outer coil is better than that of the inner coil, so for high-risk samples, external coils, such as virus banks, are generally used. The inner coil is better than the outer coil in terms of thread tightness and silicone gasket, so for cryotubes that need to be infiltrated into liquid nitrogen, many use inner coils, such as tissue samples from tissue biobanks. The wall of the cryotube is thicker than the centrifuge tube, and the manufacturing process is relatively complicated. Therefore, the cost of the cryotube is higher than that of the centrifuge tube. Since the quality of some imported centrifuge tubes is reliable, it can be used under -80 degrees, so domestic users are saving blood. At the time of the sample, many were stored at -80 °C using a 0.5-1.5 ml centrifuge tube. The capacity of the centrifuge tube is mainly determined by the capacity of the sample tube. The 1.5ml centrifuge tube can be equipped with a standard 9*9 size 2 inch freezer box, while the 0.5ml centrifuge tube can achieve 10*10, high energy. Zoom out to 2/3.
The large-scale sample library in the world is mainly based on the preservation of frozen tubes. With the upgrade of the accuracy of the analytical instrument, the sample size required for each analysis is reduced. At present, the major suppliers of consumables in the world have begun to promote micro-frozen tubes, which have a capacity of 0.5-1.0 ml and a reduced diameter. It is small enough to fit the aperture size of a 96-well plate and a 96-well cryostat. It is suitable for future mechanized sample library operations. Due to the newer products, the current application is not very wide. From the point of view of number identification, it is mainly based on manual writing marks, and some larger sample banks use barcode schemes. The labeling scheme is a way that many sample libraries hope to be realized. The implementation of barcode management must be combined with the computer system. The meaning of a simple barcode label is not great.
Software factors and cost factors are involved in the small sample library. At present, there are not many sample libraries for real application of barcodes in China. Large array sample libraries, due to many problems of manual identification, must use barcode management. The large-scale sample libraries in the world basically adopt barcode management schemes. Bar code management first involves the numbering rule problem. There is currently no very uniform rule, but basically it is based on the principle of uniqueness and sharing. Barcode management also involves professional labeling issues and should be chosen for reliable low temperature special labels. Barcode management is also a set of computer management systems for bar codes that can generate tags and corresponding bar code shortcut processing interfaces. The code system of the bar code, because the data capacity of the two-dimensional code is large, and the error correction is good, so the internationally recommended agencies are all two-dimensional bar codes. The sample library of the robot arm adopts the method of prefabricating the two-dimensional code at the bottom of the cryotube.
From the perspective of data management, most of them still stay in the era of simple database (EXCEL, ACCESS). Some sample libraries use large-capacity databases developed independently and interface with large databases. Sample information management is a very important part of the sample library. Since the overall start is relatively late, there are not many companies that do the sample library, and the basic development is mainly special development. Therefore, it has not been formed in the past several years. Sample library software. Several large sample libraries in the world basically adopt the self-developed sample library system, or a combination of several professional databases. For example, the sample library in UK Biobank is divided into a combination of a sample library platform and a laboratory data management platform. In large array research, there is also the involvement of information consulting companies such as IBM.
From the point of view of information sharing, most of the sample repositories currently use the independent information registration and setting of each unit. There is no sharing mechanism, and some are based on multi-region sharing within the scope of the project. In general, the establishment of a public platform and the development of a sharing mechanism are the mainstream of the development of the sample library. The exchange and sharing of samples is an important part of maximizing the efficiency of the sample, meeting the needs of large sample sizes, and maximizing sample utilization. The construction of the sample library is undergoing a process from individual governance to resource integration. Internationally, some large-scale integration programs such as the Pan-EU Biobanks Program (BBMRI) in Europe and the CAHUB Program in the United States have also been applied to some international organizations to actively carry out the sharing and integration of sample libraries, such as ISBER. China has also done a lot of work in this area, such as the China Human Genetic Resources Database Platform, the China Biotechnology Association's Organization of Biological Sample Bank Committee, etc. There are still many details to be carried out.

Annex B. Partial physical map

Identification application case

Annex C.
Biological Sample Library Numbering Rule Recommendation

Specimen library number naming rules:
General principle: uniqueness, shared auxiliary principle: on the basis of the general principle, try to be as simple as possible. Keep the number length as uniform as possible.
one. Organize the numbering rule organization numbering rules for biological sample collection and routine collection.

Organization numbering rule

Label type: BRADY THT-163-499-3-IP Rectangular shape is 25.4mm*9.525mm, round diameter 9.525mm
[The tissue sample number is named 1012345D08TF1, which represents the tumor sample of the D08 organ collected by the 1012345 disease (T tumor, P cancer, N normal, M metastasis, L lymph node, Y polyp, C capsule wall) in F mode (Frozen The first copy of Tissues/OCT/RNAlater/DNA/Paraffin Embedding, etc.). 】

Peripheral blood numbering rules

Label type: BRADY THT-163-499-3-IP Rectangular shape is 25.4mm*9.525mm, round diameter 9.525mm
[The blood sample number is named 1012345D19BP1, which represents the P plasma of the B blood sample of D19 organ cancer collected by the 1012345 disease (Plasma/ Serum / White Blood Cells/ Erythrocyte/Thrombolytic / DNA/ RNA/whole Blood/etc.) One. 】

Remaining cell/body fluid numbering rules

[If cord blood is a routine sample, in order to distinguish between routine peripheral blood, a sample type can be set up for cord blood, and the classification can refer to the classification of peripheral blood.

three. When the genetic database is collected by family, the recommended numbering rules are as follows

In the relationship class, P1 is the original generation of the original generation, and the rest of the relationship in the family, please define the relevant numbering rules. The first 7 sample numbers are the same individual, and the different individuals of the same individual have the same basic information. of.

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