The cultivation of unicellular algae is the basis of artificial breeding of loach. The variety, quality and quantity of monocyanobacterial bait culture directly affect the survival and growth of gonads, larvae and juveniles. According to the different stages of development of muddy snake larvae, different species of bait should be cultured and fed. We have discussed this issue. Parents were cultured in groups 1 and 4, with the other two groups having late gonads and slow gonadal development. 2.2 Effect of bait density on larval growth The D-young, transfer-to-culture, and culture densities of pro-shell oyster production after hatching were 8.11/mI and 15.17/mI, respectively. Change the water twice a day 30%, 30% each time, regular antibiotics to prevent disease, keep the water EDTA 5x10 "concentration, culture water temperature 29 °C, specific gravity 1.016-1.020. After changing the water, gold algae, results see 2. From Table 2 shows that seedlings with a bait density in the range of 50,000 to 150,000 seeds per mL show rapid growth, short metamorphic times, and high survival rates. 2.4 The effect of different bait density on the growth of juveniles was tested with three different densities of G. occidentalis and Platymonas subtilis. The first group was: 100,000 G. algae/mI + 10,000 flat algae/mL; the second group: G. 200,000/mL + Platymonas 20,000 -30000/mL; Group 3: 300,000/mL algae + 50,000 algae/mL. The results are shown in Table 4. The results showed that under the same conditions, the second group had the best results; the first group had the lowest growth rate and weight gain rate, and there was a small amount of juvenile death (see shell), and the activity was weaker.
Peeled Black Garlic is peel them first, and then fermentation,It's easy to eat.
We know that garlic itself is a very good health food, while the role of black garlic is really amazing. For diabetes, hypertension, high cholesterol, cancer prevention and treatment have a very significant effect.
Once you have peeled the clove it is ready to eat.Black Garlic
should have 24 months shelf life if stored in the correct conditions.
Peeled Black Garlic Peeled Black Garlic,Peeled Fermented Black Garlic,Fresh Peeled Black Garlic,Organic Peeled Black Garlic Zhucheng Tongxi Commercial And Trade Co.,Ltd. , http://www.blackgarlicgroup.com
1 Material Methods
1.1 The monotreme species are diatoms: Phaeodactylum tricornutum, small diatoms. Golden algae: Zhanjiang Dinoflagellates, 3011, Tahiti and so on. Platymonas subcordiformis: the heart-shaped Platymonas philippinarum and the Platycoda platyaenensis
1.2 The loach pro-shellfish was purchased from the natural sea area of ​​Wenzhou, Zhejiang, and the gonads have not yet developed. After the pro-husband is cultivated, the pro-stimulation is induced and the required larvae and juveniles are obtained. The test pool is cement pool of 5m x 6m1.1m.
1.3 Bait culture Algae culture: 5000 mL Erlenmeyer flasks are stationary and semi-continuous culture; Relay culture: 40L white plastic barrels are inflated with 1 semi-continuous culture; Productive culture: 20m2 cement pools are inflated and disposable cultured.
1.4 The proportion of seawater cultivated by physicochemical factors is 1.016-1.020. The temperature of diatom culture was natural temperature. The culture of dinoflagellates and Platycladus species was incubated in a plastic shed in the early stage.
1.5 The formulation of nutrients is: (1) Algal culture and relay culture formula: NaN03 100mg; VBl l00ug; KH2P04 10mg; VB120.5ug; ferric ammonium citrate 1mg; seawater 1000mL; ED-TA 20mg. Sodium silicate 50 mg was added to the diatom culture. (2) Formula for productive culture: NaN03 50 mg; ferric ammonium citrate 0.5 mg; KH2P04 5 mg; seawater 1000 ml. Sodium silicate 20 mg was added to the diatom culture.
1.6 Management A comprehensive observation of each species is performed daily to check the color, concentration, and vitality of the algae and to understand the approximate growth of the algae; microscopic examination of the expanded species ensures that the expanded species is free from contamination. Expand the seed according to the daily amount and the required variety, and use the relay culture to expand the seed with 1:1-1:3. Seed density: Chlorella 8 million - 10 million / mL, Platycladus 1 million -1.5 million / mL, diatoms 4 million -5 million / ml. 10-15 barrels per tank for productive cultivation, 4 to 5 days after expansion
2 results
2.1 Effect of different bait combinations on the development of loach and pro-gland gonads Pro-beet culture in indoor cement pools. The bottom of the pool is covered with 2-3cm of soft mud, the culture density is 0.5kg/m2, and the water is changed every other day. Each time 100 %, algal fluid with daily feed amount of 10% of total water. Culture water temperature 27 °C (early temperature rise 1 °C, to 27 °C only). After about 50 days of cultivation, the results are shown in Table 1.
2.3 Effect of different diets on growth of juveniles After larvae become abnormally attached, the high water level is raised to low water level, daily water changes
Note: *As the larvae develop from D-shaped larvae to shell-top larvae, the feeding amount gradually increases from 20,000/mL to 100,000/mL and gradually increases from 50,000/mL to 150,000/mL.
Δ is the time from the start of adhesion to the completion of all adhesion, that is, from the ninth day to the end of the adhesion on the thirteenth day. Once, each time 100%, change the water and then use EDTA 510-6 concentration, regular antibiotics, incubation water temperature 29 °C, the proportion of seawater 1.016-1.020, daily feeding golden algae, Platymonas. The results are shown in Table 3. From Table 3, it can be seen that the growth rate and daily gain rate of Platycladiella monocytogenes are lower than those of other species.
3 Discussion
3.1 The development of pro-occlusion gonads is directly related to seedling production. Feeding diatoms at the pro-beehive stage is best assisted with gold algae, which is conducive to pro-promoting of pro-scallops, which is consistent with natural conditions. At the same time, due to the low temperature at that time, diatoms were also easy to cultivate. In addition, it was found in experiments that after large amounts of Platymonas subtilis were inoculated, Platymonas subtilis was settled at the bottom of the pool at night and metamorphosed easily to form a dead algae mass, which caused the bottoms to be black and stink, affecting the viability of pro-shellfish.
3.2 Based on relevant data and previous work experience, the diet of loach larvae is dominated by gold algae. The test was not fed Plankella. The test results showed that the medium and high density of the golden algae is better, that is, the initial bait density of D-shaped larvae is controlled at 50,000-80,000/mL, especially in the first two days of D-age: the early shell period is 80,000- 100,000/mL; late shell project is 100,000 to 150,000/mL. The larvae have fast growth rate, high survival rate, short metamorphic attachment time, and neat specifications. If the bait density is too low, the larvae grow slowly, the specifications are uneven, and the metamorphic attachment time is long, affecting the survival rate of the larvae.
3.3 During the nurturing period, bait is one of the major factors affecting its growth and yield. The results showed that the use of gold algae and a small amount of flat algae as well, and the density of gold algae need to be maintained at 200,000 / m1, flat 20,000 algae. With 30,000/m1, the daily growth and daily weight gain of juveniles have increased significantly. If single feeding of Platycladi, the growth of juveniles is slow and their vigor is poor.
3.4 In practical breeding, single-cell algae culture is a key to success. Especially in southern Jiangsu, due to bad weather with continuous rainy weather from June to July, coupled with another high temperature season, to ensure that there is a sufficient amount of bait, especially golden algae, we must increase the relay culture volume to maintain the species of Chrysochlor 3 - 4 in case of pollution and degradation of Chlorella species. In addition, due to the high temperature and pollution resistance of Platycladus is stronger than that of Chrysophytes, the amount of culture of Platymonas can be appropriately increased to prevent accidents.
