The incubator is widely used in the research and production of medicine, immunology, genetics, microbiology, agricultural science, and pharmacy. It has become one of the most commonly used routine instruments in the above-mentioned fields, and it is formed by simulating in the incubator. Similar cell/tissue growth environment in vivo such as constant pH (pH: 7.2-7.4), stable temperature (37 ° C), high relative humidity (95%), in vitro culture of cells / tissues a device. The user has two basic requirements for the incubator. One is to require the incubator to provide the most accurate and stable control of temperature and humidity, so as to facilitate the progress of its research work. The second is to require the incubator to be able to treat the microorganisms in the incubator. Pollution is effectively prevented and pollution can be eliminated regularly to protect research results and prevent sample loss. First, temperature control 2. Temperature control system: 3. Temperature uniformity: Third, the relative humidity: Fourth, anti-pollution design and disinfection and sterilization system: V. Other factors <br>The volume of the incubator is also a factor that cannot be ignored. It is not enough to buy small, and it is wasteful and takes up space. The optional volume of the incubator is very wide and each type has a different volume. At this point, you need to have a more accurate understanding of the range of required incubator volume before purchase, and reserve a little space on this basis to ensure that it is needed. Sports Accessories,High Quality Sports Accessories,Sports Accessories Details, CN Eastony Industries (NingBo) Co., LTD. , https://www.eastony.com
1. Heating method:
Both air jacketed and water jacketed, both heating systems are accurate and reliable, and they all have their own advantages and disadvantages. Water jacket heating maintains a constant temperature by enclosing an internal tank through a separate water jacket. The advantage is that water is a good thermal insulation material. When a power failure occurs, the water jacket system can Maintaining temperature accuracy and stability in the incubator for a long period of time (maintaining a constant temperature for 3-4 times that of a gas-tight system) is beneficial to the experimental environment is not stable (such as useful electrical limits, or frequent power outages) ) and users who need to maintain stable culture conditions for a long time. The gas-sleeve heating is to directly heat the inner tank through the heaters in the gas jacket of the tank, and is also called direct heating on six sides. Compared with the water jacket type, the gas-sleeve type has the characteristics of quick heating and rapid recovery of the temperature than the water-type incubator, which is particularly advantageous for short-term cultivation and the need for frequent switching of the door. In addition, the air-sleeve design is simpler for the user than the water jacket type (water jacket type requires watering, emptying and cleaning of the water tank, and often monitoring the operation of the water tank).
Maintaining a constant temperature in the incubator is an important factor in maintaining healthy cell growth, so an accurate and reliable temperature control system is an integral part of the incubator. The user selects an incubator with independent triple temperature control functions, namely temperature control inside the box, over temperature alarm control and ambient temperature monitoring for the stable operation of the incubator.
The uniformity of temperature in the incubator is also a major factor that users need to consider. Generally, the uniformity of the incubator equipped with a fan and a duct in the cabinet is much better, and the device also contributes to the rapid temperature and relative humidity inside the box. restore.
The humidity inside the box is a very important but often overlooked factor for the training work. Maintaining sufficient humidity levels and having a fast enough humidity recovery rate (such as after opening and closing the door) will ensure that the culture fails without excessive drying. When purchasing the incubator, the user should try to choose the incubator with large humidity evaporation area, because we know that the larger the evaporation area of ​​the humidity, the easier it is to reach the maximum relative saturation humidity and the shorter the recovery time after the door is opened and closed.
The entire inner bottom of the Esun (Yi Shang) brand PY series incubator is designed as a reservoir-type chassis, so that the evaporation area can be maximized and the evaporation effect can be enhanced. The reservoir-type chassis design can quickly reach the saturated humidity state in the incubator. And the humidity recovery speed is much higher than the incubator that uses the water tray to humidify.
Contamination is a major contributor to cell culture failure, and manufacturers of incubators have designed a variety of different devices to reduce and prevent contamination, primarily by minimizing areas and surfaces where microbes can grow, and Combined with the automatic elimination of pollution devices to effectively prevent the occurrence of pollution. Such as incubator with UV disinfection function (such as Esun (Easy) PY series incubator); some design and production of HEPA filter can filter the air inside the incubator, can remove 99.97% of particles above 0.3 microns; In addition, an incubator has been developed to enable the environment inside the box to reach a high temperature and humidity to kill contaminating microorganisms and achieve sterilization and sterilization purposes. These devices are essential for cell culture, but which cleaning device is chosen? First of all, we are considering the sterilization ability of various methods. The UV disinfection ability is inversely proportional to the square of the distance from the UV lamp. The farther the distance is, the worse the disinfection ability is. Therefore, the UV disinfection method has its limitations. It is difficult to achieve the requirement of thorough sterilization; in comparison, high-temperature disinfection is currently a more effective method of disinfection and sterilization. High-temperature disinfection is divided into two categories, one is traditional high-temperature dry heat disinfection, and the other is advanced high temperature. Damp heat sterilization.
Next, we will focus on the advantages and disadvantages of high temperature dry heat and high temperature wet heat. High-temperature damp heat has high latent heat and strong penetrating power, which tends to denature or solidify proteins. Therefore, the sterilization efficiency of this method is higher than that of dry heat sterilization. There are three reasons for this: 1 The temperature required for protein coagulation is related to its water content. The higher the water content, the lower the temperature required for solidification to occur. The moist heat-sterilized bacterial protein absorbs moisture, so it is easy to coagulate in dry hot air at the same temperature. 2 During the moist heat sterilization process, the steam releases a large amount of latent heat, which accelerates the humidity. Thus, moist heat sterilization is lower than the temperature required for dry heat, and at the same temperature, the time required for moist heat sterilization is shorter than dry heat. 3 The penetration of damp heat is larger than that of dry heat, so that the deep part can also reach the sterilization temperature, so the damp heat is better than the dry heat. Therefore, high temperature sterilization is not a simple look at the disinfection temperature, mainly to see if it is hot and humid.
In addition, each user wants the instrument to be easy to use, the microprocessor control system and various other functional accessories (such as high temperature auto-tuning and alarm devices, password protection settings, automatic calibration systems, LCD display systems, etc.) The use of the incubator makes the operation and control of the incubator very simple.