Mushroom inoculation room aseptic operation and air disinfection method

Pleurotus ostreatus, shiitake mushrooms, golden mushroom mushrooms, coprinus comatus, mushroom, mushroom, and other mushrooms are all traditional food materials. They have always been known as “tasty and nutritious” Zhucheng. In recent years, the demand for mushrooms has increased and the country has consumed 10 million tons of mushrooms each year. As a result, the growing enthusiasm of mushroom growers has become higher and higher, but due to improper asepsis and air sterilization methods, the quality of mushrooms has not changed. Good or low yield.

In the case of mushrooms, microorganisms other than those required to be cultured are collectively referred to as "hybrids". If bacteria are infected during the growth of mushrooms, the bacteria will rapidly multiply, compete with the mushroom for nutrients and space, and even secrete toxins or parasites. Mushrooms, affect the normal growth and development of mushrooms, resulting in economic losses to production. Therefore, in the cultivation and cultivation of mushrooms, it should be ensured that the strains are excellent and non-hybrid, and they must establish strict sterility concepts during the inoculation process, master and use disinfection and sterilization techniques, and prevent the contamination of bacteria, thereby reducing microorganisms. Effects on the normal growth and development of mushrooms.

According to Ms. Mao Shan, a manager of Shanghai Kangjiu Disinfection Technology Co., Ltd., which specializes in the research and development of food sterilization technology and equipment manufacturing, the sterility requirements for the culture medium, tools, and environment are different due to the different types of mushrooms. Therefore, different methods, such as sterilization, disinfection and preservation, can be selected according to production conditions.

Sterilization refers to the use of physical or chemical methods to completely destroy materials, containers, appliances and all microorganisms in the air, including the vegetative bodies and dormant bodies of microorganisms, to make the materials sterile. Disinfection is the use of physical or chemical methods to kill or remove parts of the micro-organisms in the substrate, on surfaces, and in the environment. Sterilization is a mechanical method (such as filtration, centrifugation, NICOLER dynamic sterilization technology, etc.) to remove microorganisms in liquids or gases. Anti-corrosion is a technique used to prevent or inhibit the growth and reproduction of microorganisms and is a bacteriostatic process. Sterilization generally refers to the killing of microbial cells, which usually does not include spores. The agents that have this effect are called fungicides.

Ms. Mao Shan continued: Sterilization and sterilization is an important guarantee for eliminating the interference of bacteria and creating a clean and growing environment for mushrooms. It is also a basic technology in mushroom production and a key link in the success or failure of mushroom production. They are:

First, sterile operation

1. The inoculation room should be kept clean, scrub the countertops and walls with pulverized coal phenol soap and fumigate regularly with lactic acid or formaldehyde. Before each use, UV lamps are used to sterilize; during use, “NICOLER” dynamic sterilization machine is used. This machine is turned on when the workers go to work, continuous sterilization is not allowed during the process, and the workers are turned off after work and are harmless to the human body. At the same time, the bacteria in the air, Bacillus subtilis, Salmonella and other bacteria are killed in the air.

2, before entering the inoculation room, should do a good job of personal hygiene, in the buffer room to replace the work shoes and hats, work clothes, wear masks. Work clothes, work shoes, and masks are only allowed in the inoculation room. Do not wear to other places, and regularly replace and disinfect.

3, inoculated test tubes, triangle and other bottles should be marked, indicating the name of the culture medium, bacteria, date. All items moved into the inoculation room must be wiped clean with 70% alcohol in the buffer chamber.

4. Before inoculation, both hands use an automatic hand sterilizer for hand disinfection. It is recommended that 75% medical alcohol be used as a sterilizing medium process: “induction soap hand washing—faucet rinsing—inductive drying—inductive hand disinfection”; Calculate the time after the first sterilization, and then re-disinfect the hands every 60-90 minutes to block the breeding and reproduction of bacteria on the hand.

5. The incubator and inoculation tools should be cleaned and disinfected frequently; the flame of the alcohol lamp should not leave the operation process; the tampon should not be placed indiscriminately; the inoculation tool must be sterilized before and after use.

Second, the inoculation room air disinfection method

1, UV disinfection method, the bactericidal mechanism is that when it acts on the organism, it can cause the photochemical reaction of the nucleic acid and enzyme in the cell and cause the cell to die. In addition, ultraviolet rays can also cause ozone in the air to produce ozone, which has a bactericidal effect.

However, the effect on fungi is poor, and it only serves as a supplementary disinfection, and it needs to be used in conjunction with drugs. Due to the harmful effects of UV on the human body, do not work with the UV light turned on.

2. The ozone generator can efficiently and quickly kill various microorganisms in the air and on the surface of objects. The success rate of inoculation can reach more than 97%. At the same time, it has the characteristics of stable performance, simple operation and low power consumption. It is harmful to the human body. Do not work under someone's circumstances.

3. “NICOLER Dynamic Air Sterilizer” sterilizes the air in the workshop to ensure food safety. When the disinfection technology is used to disinfect the workshop, people can work in the workshop at the same time without any harm to the human body, and at the same time keep the controlled environment in the "sterile and dust-free" standard.

4, formaldehyde and potassium permanganate fumigation method, the use of method is to use 8-10mL 40% formaldehyde and 5-7g potassium permanganate per cubic meter of space, first poured potassium permanganate into a ceramic or glass container, then add Formaldehyde; Immediately after the addition of formaldehyde to leave, 20-30 minutes when closed rooms are sterilized.

Third, the inoculation room sterility inspection method

Take a sterile nutrient agar plate, place a set on the inoculation table and under the table, open the lid for 15 minutes, and then cover it, and incubate at 37°C for 24 to 28 hours, if the colony does not exceed 4 in each dish One can think that the degree of sterility is good. If there are many colonies, the inoculation room should be further sterilized.

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