The BPAP System makes a good performance in Neonatal Intensive Care Units (NICU) and other departments. Offer you the choices you need in ventilation, monitoring and technique. what`s more, our expertise in ventilation is based on rich 23-year history of developing BPAP solutions that meet your needs. Good performance based on High Security, High Accuracy, High Stability and accurate monitoring. BPAP 30,Ventilator Non Invasive,Non Invasive Ventilation Equipment,Non Invasive Vent Equipment Shenyang RMS MEDICAL TECH CO.,LTD , https://www.medicalrms.com
FITC labeled antibody - Chadwick's method
FITC labeled antibody - Chadwick's method
       Â
Reagents:
Antibody globulin solution, fluorescein isothiocyanate, 3% sodium bicarbonate aqueous solution, 0.01 mol/L PBS pH 8.0, 1% thimerosal;
material:
Centrifuges and centrifuge tubes, flasks (25ml), ice troughs, sterile pipettes and capillary drip tubes, dialysis bags, glass rods, cotton threads and beakers (500ml);
Method and steps:
1. Preparation of antibodies. The concentration of the immunoglobulin solution was diluted to 30-40 mg/ml with PBS at pH 8.0 at 0-4 ° C, placed in an Erlenmeyer flask, and placed in an ice bath;
2. Preparation of fluorescein. Calculate the amount of fluorescein required per mg of immunoglobulin plus 0.01 mg of fluorescein, and dissolve it with 3% sodium bicarbonate solution;
3. Mix the prepared antibody and the fluorochrome solution in equal amounts, stir well, and combine it in the refrigerator at 0-4 ° C for 18-24 hours;
4. Dialysis. After the combination of fluorescein and globulin, the globulin solution was centrifuged at 2500 r/min for 20 min to remove a small amount of the precipitate, which was then placed in a dialysis bag and placed in a beaker at pH 8.0 at 0-4 °C. Dialysis of the buffered saline solution overnight;
5. Pass the column. The dialyzed overnight label was passed through a glucose gel G-25 or G-50 column to separate the free fluorescein, and the labeled fluorescent antibody was collected for identification;