The ABI7500 fluorescence quantitative PCR instrument equipped with Miki Biotechnology is an integrated platform for the detection and quantification of specific target genes. The 7500 combines PCR thermal cycling, fluorescence detection and various application analysis software to quantitatively observe the amplification products of each cycle of PCR in each reaction tube . After the end of the PCR reaction, quantitative results can be obtained without the need for gel electrophoresis analysis, PCR product purification or any other experimental procedure. The 7500 real-time PCR instrument has the advantages of time saving, high sensitivity, high accuracy and avoidance of product contamination. ABI 7500 fluorescence quantitative PCR instrument
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ABI7500 real-time PCR and its application
Real-time PCR principles
Real-time quantitative PCR is a method in which a fluorescent group is added to a PCR reaction system, and the whole PCR process is monitored in real time by using fluorescence signal accumulation, and finally the unknown template is quantitatively analyzed by a standard curve. In the ABI7500 fluorescent quantitative PCR reaction system, an excess of SYBR fluorescent dye was added. When the SYBR fluorescent dye specifically incorporates the DNA double strand, a fluorescent signal is emitted, and the SYBR dye molecule not incorporated into the DNA strand does not emit any fluorescent signal, thereby ensuring an increase in the fluorescent signal and an increase in the PCR product. Synchronization .
Technical advantages
1. Computer chart PCR amplification method, filled in table PCR programming, program running can flash the current status.
2. The sample base is basically configured as a replaceable 96-well pedestal, which can meet different PCR reaction tubes, different number of holes and different types of PCR experiments.
3. The computer calculates the actual temperature of different volume samples, and the temperature control is accurate, and the amplification effect is more reproducible.
4. The software has a large capacity, can store 100 complete PCR methods, and can automatically calculate the primer melting temperature.
5. Automatic power-off protection, after the power is restored, the unfinished cycle can be automatically executed to ensure the safe operation of the whole process of amplification.
Gene expression analysis
2. Mutation detection
3. Detection of pathogenic microorganisms such as bacteria and viruses
4. Food hygiene quarantine, etc.